Human S1PR4 ELISA Kit

Alternative name
Human S1P receptor 4 ELISA Kit;Human S1P4 ELISA Kit;Human endothelial differentiation G-protein coupled receptor 6 ELISA Kit;Human sphingosine 1-phosphate receptor Edg-6 ELISA Kit;Human S1P receptor Edg-6 ELISA Kit;Human EDG6 ELISA Kit;Human LPC1 ELISA Kit;Human SLP4 ELISA Kit;Human sphingosine-1-phosphate receptor 4 ELISA Kit;Human sphingosine 1-phosphate receptor 4 ELISA Kit;Human endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 6 ELISA Kit;
Catalog
E036444
species
Human
GeneS1PR4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 75pg/ml
Intended UseHuman S1PR4 ELISA Kit allows for the in vitro quantitative determination of S1PR4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human S1PR4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human S1PR4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human S1PR4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human S1PR4 can be calculated.
Human Sphingosine 1-phosphate receptor 4 Protein information
Uniprot ID S1PR4_HUMAN
Uniprot AC O95977; D6W612;
UniGene Hs.662006;
GeneID 8698
KEGG hsa:8698;
Human Sphingosine 1-phosphate receptor 4 Protein SEQUENCE
SEQUENCE 384 AA; 41623 MW; 369A7BC56AB46A47 CRC64;
MNATGTPVAP ESCQQLAAGG HSRLIVLHYN HSGRLAGRGG PEDGGLGALR
GLSVAASCLV VLENLLVLAA ITSHMRSRRW VYYCLVNITL SDLLTGAAYL
ANVLLSGART FRLAPAQWFL REGLLFTALA ASTFSLLFTA GERFATMVRP
VAESGATKTS RVYGFIGLCW LLAALLGMLP LLGWNCLCAF DRCSSLLPLY
SKRYILFCLV IFAGVLATIM GLYGAIFRLV QASGQKAPRP AARRKARRLL
KTVLMILLAF LVCWGPLFGL LLADVFGSNL WAQEYLRGMD WILALAVLNS
AVNPIIYSFR SREVCRAVLS FLCCGCLRLG MRGPGDCLAR AVEAHSGAST
TDSSLRPRDS FRGSRSLSFR MREPLSSISS VRSI
UCSC uc002lxg.4; human.;

Category

Human S1PR4 ELISA Kit