Human SCP2 ELISA Kit

Alternative name
Human NSL-TP ELISA Kit;Human propanoyl-CoA C-acyltransferase ELISA Kit;Human SCP-CHI ELISA Kit;Human SCPX ELISA Kit;Human sterol carrier protein 2 ELISA Kit;Human SCP-2 ELISA Kit;Human sterol carrier protein X ELISA Kit;Human SCP-X ELISA Kit;Human NLTP ELISA Kit;Human non-specific lipid-transfer protein ELISA Kit;
Catalog
E036732
species
Human
GeneSCP2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman SCP2 ELISA Kit allows for the in vitro quantitative determination of SCP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This SREBP ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SREBP in the sample, this SREBP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SREBP concentration. The concentration of SREBP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This SREBP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for SREBP. Standards or samples are then added to the microtiter plate wells and SREBP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SREBP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SREBP are added to each well to "sandwich" the SREBP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain SREBP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Human Non-specific lipid-transfer protein Protein information
Uniprot ID NLTP_HUMAN
Uniprot AC Q15432; Q16622; Q5VVZ1; Q6NXF4; Q99430;
UniGene Hs.476365;
GeneID 6342
KEGG hsa:6342;
Human Non-specific lipid-transfer protein Protein SEQUENCE
SEQUENCE 547 AA; 58994 MW; 29F7551465C7143A CRC64;
MSSSPWEPAT LRRVFVVGVG MTKFVKPGAE NSRDYPDLAE EAGKKALADA
QIPYSAVDQA CVGYVFGDST CGQRAIYHSL GMTGIPIINV NNNCATGSTA
LFMARQLIQG GVAECVLALG FEKMSKGSLG IKFSDRTIPT DKHVDLLINK
YGLSAHPVAP QMFGYAGKEH MEKYGTKIEH FAKIGWKNHK HSVNNPYSQF
QDEYSLDEVM ASKEVFDFLT ILQCCPTSDG AAAAILASEA FVQKYGLQSK
AVEILAQEMM TDLPSSFEEK SIIKMVGFDM SKEAARKCYE KSGLTPNDID
VIELHDCFST NELLTYEALG LCPEGQGATL VDRGDNTYGG KWVINPSGGL
ISKGHPLGAT GLAQCAELCW QLRGEAGKRQ VPGAKVALQH NLGIGGAVVV
TLYKMGFPEA ASSFRTHQIE AVPTSSASDG FKANLVFKEI EKKLEEEGEQ
FVKKIGGIFA FKVKDGPGGK EATWVVDVKN GKGSVLPNSD KKADCTITMA
DSDFLALMTG KMNPQSAFFQ GKLKITGNMG LAMKLQNLQL QPGNAKL
UCSC uc001cuq.3; human. [P22307-1];

Category

Human SCP2 ELISA Kit