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Alternative name
Stomatin-like protein 2, mitochondrial ELISA KIT; EPB72-like protein 2 ELISA KIT; Paraprotein target 7 ELISA KIT; Paratarg-7STOML2 ELISA KIT; SLP2 ELISA KIT; HSPC108 ELISA KIT; SLP-2 ELISA KIT; Paratarg-7 ELISA KIT
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Catalog
E036763
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species
Human
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GeneSLP 2
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman SLP 2 ELISA Kit allows for the in vitro quantitative determination of SLP 2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalPrinciple of the Assay: SLP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLP-2 antibody and an SLP-2-HRP conjugate. The assay sample and buffer are incubated together with SLP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLP-2 concentration since SLP-2 from samples and SLP-2-HRP conjugate compete for the anti-SLP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLP-2 from the sample, fewer sites are left to bind SLP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLP-2 concentration in each sample is interpolated from this standard curve.
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