Human SDF2 ELISA Kit

Alternative name
Human SDF-2 ELISA Kit;Human stromal cell derived factor 2 ELISA Kit;Human stromal cell-derived factor 2 ELISA Kit;
Catalog
E036803
species
Human
GeneSDF2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman SDF2 ELISA Kit allows for the in vitro quantitative determination of SDF2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: SDF2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDF2 Standards or samples are then added to the microtiter plate wells and SDF2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDF2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for SDF2 are added to each well to "sandwich" the SDF2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDF2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF2 concentration in each sample is interpolated from this standard curve.
Human Stromal cell-derived factor 2 Protein information
Uniprot ID SDF2_HUMAN
Uniprot AC Q99470; Q9BQ79;
UniGene Hs.514036;
GeneID 6388
KEGG hsa:6388;
Human Stromal cell-derived factor 2 Protein SEQUENCE
SEQUENCE 211 AA; 23026 MW; 5EFC91C62E7B61E9 CRC64;
MAVVPLLLLG GLWSAVGASS LGVVTCGSVV KLLNTRHNVR LHSHDVRYGS
GSGQQSVTGV TSVDDSNSYW RIRGKSATVC ERGTPIKCGQ PIRLTHVNTG
RNLHSHHFTS PLSGNQEVSA FGEEGEGDYL DDWTVLCNGP YWVRDGEVRF
KHSSTEVLLS VTGEQYGRPI SGQKEVHGMA QPSQNNYWKA MEGIFMKPSE
LLKAEAHHAE L
UCSC uc002hbw.4; human.;

Category

Human SDF2 ELISA Kit

Human SDF-2 ELISA Kit;Human stromal cell derived factor 2 ELISA Kit;Human stromal cell-derived factor 2 ELISA Kit;