Human STXBP1 ELISA Kit

Alternative name
Human MUNC18-1 ELISA Kit;Human N-Sec1 ELISA Kit;Human protein unc-18 homolog 1 ELISA Kit;Human unc18-1 ELISA Kit;Human protein unc-18 homolog A ELISA Kit;Human unc-18A ELISA Kit;Human P67 ELISA Kit;Human UNC18A ELISA Kit;Human NSEC1 ELISA Kit;Human RBSEC1 ELISA Kit;Human UNC18 ELISA Kit;Human syntaxin binding protein 1 ELISA Kit;Human syntaxin-binding protein 1 ELISA Kit;Human neuronal SEC1 ELISA Kit;
Catalog
E037227
species
Human
GeneSTXBP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of STXBP1. No significant cross-reactivity or interference between STXBP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STXBP1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman STXBP1 ELISA Kit allows for the in vitro quantitative determination of STXBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalIntended Uses: This STXBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STXBP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||STXBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-STXBP1 antibody and an STXBP1-HRP conjugate. The assay sample and buffer are incubated together with STXBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STXBP1 concentration since STXBP1 from samples and STXBP1-HRP conjugate compete for the anti-STXBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by STXBP1 from the sample, fewer sites are left to bind STXBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STXBP1 concentration in each sample is interpolated from this standard curve.
Human Syntaxin-binding protein 1 Protein information
Uniprot ID STXB1_HUMAN
Uniprot AC P61764; B1AM97; Q28208; Q62759; Q64320; Q96TG8;
UniGene Hs.288229;
GeneID 6812
KEGG hsa:6812;
Human Syntaxin-binding protein 1 Protein SEQUENCE
SEQUENCE 594 AA; 67569 MW; 2DD0715F875CE0F3 CRC64;
MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI
MTEGITIVED INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR
AAHVFFTDSC PDALFNELVK SRAAKVIKTL TEINIAFLPY ESQVYSLDSA
DSFQSFYSPH KAQMKNPILE RLAEQIATLC ATLKEYPAVR YRGEYKDNAL
LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS SPVLHELTFQ
AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS
QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL
AEDCMKHYQG TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS
TYDKIRIILL YIFLKNGITE ENLNKLIQHA QIPPEDSEII TNMAHLGVPI
VTDSTLRRRS KPERKERISE QTYQLSRWTP IIKDIMEDTI EDKLDTKHYP
YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF ILGGVSLNEM
RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS
UCSC uc004brk.2; human.;

Category

Human STXBP1 ELISA Kit