SpecificityThis assay has high sensitivity and excellent specificity for detection of THY1. No significant cross-reactivity or interference between THY1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between THY1 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman THY1 ELISA Kit allows for the in vitro quantitative determination of THY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntended Uses: This THY1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human THY1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: THY1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-THY1 antibody and an THY1-HRP conjugate. The assay sample and buffer are incubated together with THY1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the THY1 concentration since THY1 from samples and THY1-HRP conjugate compete for the anti-THY1 antibody binding site. Since the number of sites is limited, as more sites are occupied by THY1 from the sample, fewer sites are left to bind THY1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The THY1 concentration in each sample is interpolated from this standard curve.
Human Thy-1 membrane glycoprotein Protein information
Uniprot ID
THY1_HUMAN
Uniprot AC
P04216; Q16008; Q9NSP1;
UniGene
Hs.644697;
GeneID
7070
KEGG
hsa:7070;
Human Thy-1 membrane glycoprotein Protein SEQUENCE