Intended UseHuman TSLP ELISA Kit allows for the in vitro quantitative determination of TSLP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description specificalPrinciple of the Assay: TSLP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TSLP antibody and an TSLP-HRP conjugate. The assay sample and buffer are incubated together with TSLP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TSLP concentration since TSLP from samples and TSLP-HRP conjugate compete for the anti-TSLP antibody binding site. Since the number of sites is limited, as more sites are occupied by TSLP from the sample, fewer sites are left to bind TSLP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSLP concentration in each sample is interpolated from this standard curve.
Human Thymic stromal lymphopoietin Protein information
Uniprot ID
TSLP_HUMAN
Uniprot AC
Q969D9; G3XAM8; Q8IW99; Q96AU7;
UniGene
Hs.389874;
GeneID
85480
KEGG
hsa:85480;
Human Thymic stromal lymphopoietin Protein SEQUENCE