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Human TMEM127 ELISA Kit

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  • Alternative name

    Human transmembrane protein 127 ELISA Kit;
  • Catalog
    E038450
  • species
    Human
  • GeneTMEM127
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TMEM127 . No significant cross-reactivity or interference between TMEM127 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 46.9pg/ml
  • Intended UseHuman TMEM127 ELISA Kit allows for the in vitro quantitative determination of TMEM127 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TMEM127antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TMEM127 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TMEM127 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TMEM127 can be calculated.
  • Human Transmembrane protein 127 Protein information
  • Uniprot ID TM127_HUMAN
  • Uniprot AC O75204; D3DXH0;
  • UniGene Hs.164303; Hs.735706;
  • GeneID 55654
  • KEGG hsa:55654;
  • Human Transmembrane protein 127 Protein SEQUENCE

  • SEQUENCE 238 AA; 25842 MW; 9A351F13EA9C6865 CRC64;

    MYAPGGAGLP GGRRRRSPGG SALPKQPERS LASALPGALS ITALCTALAE

    PAWLHIHGGT CSRQELGVSD VLGYVHPDLL KDFCMNPQTV LLLRVIAAFC

    FLGILCSLSA FLLDVFGPKH PALKITRRYA FAHILTVLQC ATVIGFSYWA

    SELILAQQQQ HKKYHGSQVY VTFAVSFYLV AGAGGASILA TAANLLRHYP

    TEEEEQALEL LSEMEENEPY PAEYEVINQF QPPPAYTP

  • UCSC uc002svq.4; human.;



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