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Human TNFSF12 ELISA Kit

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  • Alternative name

    Human APO3 ligand ELISA Kit;Human TNF-related WEAK inducer of apoptosis ELISA Kit;Human TWEAK ELISA Kit;Human APO3L ELISA Kit;Human DR3LG ELISA Kit;Human TNLG4A ELISA Kit;Human tumor necrosis factor superfamily member 12 ELISA Kit;Human tumor necrosis factor ligand superfamily member 12 ELISA Kit;Human APO3/DR3 ligand ELISA Kit;Human tumor necrosis factor (ligand) superfamily, member 12 ELISA Kit;Human tumor necrosis factor ligand 4A ELISA Kit;
  • Catalog
    E038504
  • species
    Human
  • GeneTNFSF12
  • SpecificityNatural and recombinant human TNFSF12
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman TNFSF12 ELISA Kit allows for the in vitro quantitative determination of TNFSF12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human TNFSF12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF12 has been precoated onto 96-well plates. Standards(E.coli, S94-H249) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF12 amount of sample captured in plate. Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.
  • Human Tumor necrosis factor ligand superfamily member 12, secreted form Protein information
  • Uniprot ID TNF12_HUMAN
  • Uniprot AC O43508; Q8IZK7; Q8WUZ7;
  • UniGene Hs.54673;
  • GeneID 8742
  • KEGG hsa:8742;
  • Human Tumor necrosis factor ligand superfamily member 12, secreted form Protein SEQUENCE

  • SEQUENCE 249 AA; 27216 MW; E660843361C28EBA CRC64;

    MAARRSQRRR GRRGEPGTAL LVPLALGLGL ALACLGLLLA VVSLGSRASL

    SAQEPAQEEL VAEEDQDPSE LNPQTEESQD PAPFLNRLVR PRRSAPKGRK

    TRARRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEEARINS SSPLRYNRQI

    GEFIVTRAGL YYLYCQVHFD EGKAVYLKLD LLVDGVLALR CLEEFSATAA

    SSLGPQLRLC QVSGLLALRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH




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