Human TCN1 ELISA Kit

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  • Alternative name

    Human TC-1 ELISA Kit;Human haptocorrin ELISA Kit;Human HC ELISA Kit;Human protein R ELISA Kit;Human Transcobalamin I ELISA Kit;Human TC I ELISA Kit;Human TCI ELISA Kit;Human TC1 ELISA Kit;Human transcobalamin 1 ELISA Kit;Human transcobalamin-1 ELISA Kit;Human haptocorin ELISA Kit;Human transcobalamin I (vitamin B12 binding protein, R binder family) ELISA Kit;

  • Catalog
    E038748
  • species
    Human
  • GeneTCN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Transcobalamin I (TCN1). No significant cross-reactivity or interference between Transcobalamin I (TCN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.1pg/mL.
  • Intended UseHuman TCN1 ELISA Kit allows for the in vitro quantitative determination of TCN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcobalamin I (TCN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcobalamin I (TCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcobalamin I (TCN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcobalamin I (TCN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Transcobalamin-1 Protein information
  • Uniprot ID TCO1_HUMAN
  • Uniprot AC P20061; A8KAC5; Q8WV77;
  • UniGene Hs.2012;
  • GeneID 6947
  • KEGG hsa:6947;
  • Human Transcobalamin-1 Protein SEQUENCE
  • SEQUENCE 433 AA; 48207 MW; CF1052BE3DBA929A CRC64;

    MRQSHQLPLV GLLLFSFIPS QLCEICEVSE ENYIRLKPLL NTMIQSNYNR

    GTSAVNVVLS LKLVGIQIQT LMQKMIQQIK YNVKSRLSDV SSGELALIIL

    ALGVCRNAEE NLIYDYHLID KLENKFQAEI ENMEAHNGTP LTNYYQLSLD

    VLALCLFNGN YSTAEVVNHF TPENKNYYFG SQFSVDTGAM AVLALTCVKK

    SLINGQIKAD EGSLKNISIY TKSLVEKILS EKKENGLIGN TFSTGEAMQA

    LFVSSDYYNE NDWNCQQTLN TVLTEISQGA FSNPNAAAQV LPALMGKTFL

    DINKDSSCVS ASGNFNISAD EPITVTPPDS QSYISVNYSV RINETYFTNV

    TVLNGSVFLS VMEKAQKMND TIFGFTMEER SWGPYITCIQ GLCANNNDRT

    YWELLSGGEP LSQGAGSYVV RNGENLEVRW SKY

  • UCSC uc001noj.3; human.;



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