Human CTCFL ELISA Kit

Alternative name
Human brother of the regulator of imprinted sites ELISA Kit;Human CCCTC-binding factor ELISA Kit;Human CTCF paralog ELISA Kit;Human CTCF-like protein ELISA Kit;Human cancer/testis antigen 27 ELISA Kit;Human CT27 ELISA Kit;Human Zinc finger protein CTCF-T ELISA Kit;Human BORIS ELISA Kit;Human CTCF-T ELISA Kit;Human HMGB1L1 ELISA Kit;Human dJ579F20.2 ELISA Kit;Human CCCTC-binding factor like ELISA Kit;Human transcriptional repressor CTCFL ELISA Kit;Human BORIS-like protein ELISA Kit;
Catalog
E038883
species
Human
GeneCTCFL
SpecificityThis assay has high sensitivity and excellent specificity for detection of CTCFL . No significant cross-reactivity or interference between CTCFL and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.188ng/ml
Intended UseHuman CTCFL ELISA Kit allows for the in vitro quantitative determination of CTCFL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CTCFL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CTCFL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CTCFL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CTCFL can be calculated.
Human Transcriptional repressor CTCFL Protein information
Uniprot ID CTCFL_HUMAN
Uniprot AC Q9BZ30; Q9NQJ3;
UniGene Hs.131543;
GeneID 140690
KEGG hsa:140690;
Human Transcriptional repressor CTCFL Protein SEQUENCE
SEQUENCE 663 AA; 75747 MW; 9351E1A85173D085 CRC64;
MAATEISVLS EQFTKIKELE LMPEKGLKEE EKDGVCREKD HRSPSELEAE
RTSGAFQDSV LEEEVELVLA PSEESEKYIL TLQTVHFTSE AVELQDMSLL
SIQQQEGVQV VVQQPGPGLL WLEEGPRQSL QQCVAISIQQ ELYSPQEMEV
LQFHALEENV MVASEDSKLA VSLAETTGLI KLEEEQEKNQ LLAERTKEQL
FFVETMSGDE RSDEIVLTVS NSNVEEQEDQ PTAGQADAEK AKSTKNQRKT
KGAKGTFHCD VCMFTSSRMS SFNRHMKTHT SEKPHLCHLC LKTFRTVTLL
RNHVNTHTGT RPYKCNDCNM AFVTSGELVR HRRYKHTHEK PFKCSMCKYA
SVEASKLKRH VRSHTGERPF QCCQCSYASR DTYKLKRHMR THSGEKPYEC
HICHTRFTQS GTMKIHILQK HGENVPKYQC PHCATIIARK SDLRVHMRNL
HAYSAAELKC RYCSAVFHER YALIQHQKTH KNEKRFKCKH CSYACKQERH
MTAHIRTHTG EKPFTCLSCN KCFRQKQLLN AHFRKYHDAN FIPTVYKCSK
CGKGFSRWIN LHRHSEKCGS GEAKSAASGK GRRTRKRKQT ILKEATKGQK
EAAKGWKEAA NGDEAAAEEA STTKGEQFPG EMFPVACRET TARVKEEVDE
GVTCEMLLNT MDK
UCSC uc010giw.2; human. [Q8NI51-1];

Category

Human CTCFL ELISA Kit