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Alternative name
Transforming growth factor-beta-induced protein ig-h3 ELISA KIT; Kerato-epithelin ELISA KIT; RGD-containing collagen-associated protein ELISA KIT; RGD-CAPTGFBI ELISA KIT; BIGH3 ELISA KIT; Beta ig-h3 ELISA KIT; RGD-CAP ELISA KIT
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Catalog
E038967
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species
Human
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GeneTGFBI/betaIG-H3
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<46.9pg/ml
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Intended UseHuman TGFBI/betaIG-H3 ELISA Kit allows for the in vitro quantitative determination of TGFBI/betaIG-H3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human TGFBI antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human TGFBI antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFBI amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of human TGFBI can be calculated.
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