Human TMED1 ELISA Kit

Alternative name
Human Interleukin-1 receptor-like 1 ligand ELISA Kit;Human putative T1/ST2 receptor-binding protein ELISA Kit;Human p24 family protein gamma-1 ELISA Kit;Human Tp24 ELISA Kit;Human p24gamma1 ELISA Kit;Human Il1rl1l ELISA Kit;Human IL1RL1LG ELISA Kit;Human p24g1 ELISA Kit;Human transmembrane p24 trafficking protein 1 ELISA Kit;Human transmembrane emp24 domain-containing protein 1 ELISA Kit;Human IL1RL1-binding protein ELISA Kit;Human interleukin 1 receptor-like 1 ligand ELISA Kit;Human transmembrane emp24 protein transport domain containing 1 ELISA Kit;
Catalog
E039121
species
Human
GeneTMED1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman TMED1 ELISA Kit allows for the in vitro quantitative determination of TMED1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: TMED1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TMED1. Standards or samples are then added to the microtiter plate wells and TMED1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TMED1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TMED1 are added to each well to "sandwich" the TMED1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TMED1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMED1 concentration in each sample is interpolated from this standard curve.
Human Transmembrane emp24 domain-containing protein 1 Protein information
Uniprot ID TMED1_HUMAN
Uniprot AC Q13445;
UniGene Hs.211463; Hs.515139; Hs.661691;
GeneID 11018
KEGG hsa:11018;
Human Transmembrane emp24 domain-containing protein 1 Protein SEQUENCE
SEQUENCE 227 AA; 25206 MW; 058C5274E05F8575 CRC64;
MMAAGAALAL ALWLLMPPVE VGGAGPPPIQ DGEFTFLLPA GRKQCFYQSA
PANASLETEY QVIGGAGLDV DFTLESPQGV LLVSESRKAD GVHTVEPTEA
GDYKLCFDNS FSTISEKLVF FELIFDSLQD DEEVEGWAEA VEPEEMLDVK
MEDIKESIET MRTRLERSIQ MLTLLRAFEA RDRNLQEGNL ERVNFWSAVN
VAVLLLVAVL QVCTLKRFFQ DKRPVPT
UCSC uc002mpy.5; human.;

Category

Human TMED1 ELISA Kit