Human NTRK1 ELISA Kit

Alternative name
Human Neurotrophic tyrosine kinase receptor type 1 ELISA Kit;Human TRK1-transforming tyrosine kinase protein ELISA Kit;Human tropomyosin-related kinase A ELISA Kit;Human Tyrosine kinase receptor ELISA Kit;Human tyrosine kinase receptor A ELISA Kit;Human Trk-A ELISA Kit;Human gp140trk ELISA Kit;Human p140-TrkA ELISA Kit;Human MTC ELISA Kit;Human TRK ELISA Kit;Human TRKA ELISA Kit;Human TRK1 ELISA Kit;Human neurotrophic receptor tyrosine kinase 1 ELISA Kit;Human high affinity nerve growth factor receptor ELISA Kit;Human Oncogene TRK ELISA Kit;Human neurotrophic tyrosine kinase, receptor, type 1 ELISA Kit;
Catalog
E039542
species
Human
GeneNTRK1
SpecificityNatural and recombinant human TrkA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman NTRK1 ELISA Kit allows for the in vitro quantitative determination of NTRK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TrkA amount of sample captured in plate. Background: Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able toactivate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are otherneurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found inkehumanoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4The standard product used in this kit is extracellular part (A33-P418) of recombinant human TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.
Human High affinity nerve growth factor receptor Protein information
Uniprot ID NTRK1_HUMAN
Uniprot AC Q7Z5C3; Q9UIU7;
UniGene Hs.406293;
GeneID 4914
KEGG hsa:4914;
Human High affinity nerve growth factor receptor Protein SEQUENCE
SEQUENCE 796 AA; 87497 MW; 6C15C721E336B601 CRC64;
MLRGGRRGQL GWHSWAAGPG SLLAWLILAS AGAAPCPDAC CPHGSSGLRC
TRDGALDSLH HLPGAENLTE LYIENQQHLQ HLELRDLRGL GELRNLTIVK
SGLRFVAPDA FHFTPRLSRL NLSFNALESL SWKTVQGLSL QELVLSGNPL
HCSCALRWLQ RWEEEGLGGV PEQKLQCHGQ GPLAHMPNAS CGVPTLKVQV
PNASVDVGDD VLLRCQVEGR GLEQAGWILT ELEQSATVMK SGGLPSLGLT
LANVTSDLNR KNVTCWAEND VGRAEVSVQV NVSFPASVQL HTAVEMHHWC
IPFSVDGQPA PSLRWLFNGS VLNETSFIFT EFLEPAANET VRHGCLRLNQ
PTHVNNGNYT LLAANPFGQA SASIMAAFMD NPFEFNPEDP IPVSFSPVDT
NSTSGDPVEK KDETPFGVSV AVGLAVFACL FLSTLLLVLN KCGRRNKFGI
NRPAVLAPED GLAMSLHFMT LGGSSLSPTE GKGSGLQGHI IENPQYFSDA
CVHHIKRRDI VLKWELGEGA FGKVFLAECH NLLPEQDKML VAVKALKEAS
ESARQDFQRE AELLTMLQHQ HIVRFFGVCT EGRPLLMVFE YMRHGDLNRF
LRSHGPDAKL LAGGEDVAPG PLGLGQLLAV ASQVAAGMVY LAGLHFVHRD
LATRNCLVGQ GLVVKIGDFG MSRDIYSTDY YRVGGRTMLP IRWMPPESIL
YRKFTTESDV WSFGVVLWEI FTYGKQPWYQ LSNTEAIDCI TQGRELERPR
ACPPEVYAIM RGCWQREPQQ RHSIKDVHAR LQALAQAPPV YLDVLG
UCSC uc001fqf.2; human. [P04629-1];

Category

Human NTRK1 ELISA Kit