Human TPBG ELISA Kit

Alternative name
Human 5T4 oncofetal antigen ELISA Kit;Human 5T4 oncofetal trophoblast glycoprotein ELISA Kit;Human 5T4 oncotrophoblast glycoprotein ELISA Kit;Human M6P1 ELISA Kit;Human wnt-activated inhibitory factor 1 ELISA Kit;Human WAIF1 ELISA Kit;Human 5T4 ELISA Kit;Human 5T4AG ELISA Kit;Human trophoblast glycoprotein ELISA Kit;
Catalog
E039568
species
Human
GeneTPBG
SpecificityThis assay has high sensitivity and excellent specificity for detection of human TPBG. No significant cross-reactivity or interference between human TPBG and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human TPBG is typically less than 0.11 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman TPBG ELISA Kit allows for the in vitro quantitative determination of TPBG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPBG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPBG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPBG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPBG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Trophoblast glycoprotein Protein information
Uniprot ID TPBG_HUMAN
Uniprot AC Q13641; A8K555;
UniGene Hs.71947; Hs.82128;
GeneID 7162
KEGG hsa:7162;
Human Trophoblast glycoprotein Protein SEQUENCE
SEQUENCE 420 AA; 46032 MW; 3C403211F40F18BC CRC64;
MPGGCSRGPA AGDGRLRLAR LALVLLGWVS SSSPTSSASS FSSSAPFLAS
AVSAQPPLPD QCPALCECSE AARTVKCVNR NLTEVPTDLP AYVRNLFLTG
NQLAVLPAGA FARRPPLAEL AALNLSGSRL DEVRAGAFEH LPSLRQLDLS
HNPLADLSPF AFSGSNASVS APSPLVELIL NHIVPPEDER QNRSFEGMVV
AALLAGRALQ GLRRLELASN HFLYLPRDVL AQLPSLRHLD LSNNSLVSLT
YVSFRNLTHL ESLHLEDNAL KVLHNGTLAE LQGLPHIRVF LDNNPWVCDC
HMADMVTWLK ETEVVQGKDR LTCAYPEKMR NRVLLELNSA DLDCDPILPP
SLQTSYVFLG IVLALIGAIF LLVLYLNRKG IKKWMHNIRD ACRDHMEGYH
YRYEINADPR LTNLSSNSDV
UCSC uc003pjn.5; human.;

Category

Human TPBG ELISA Kit