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Alternative name
Tumor necrosis factor ligand superfamily member 4 ELISA KIT; Glycoprotein Gp34 ELISA KIT; OX40 ligand ELISA KIT; OX40L ELISA KIT; TAX transcriptionally-activated glycoprotein 1 ELISA KIT; CD_antigen: CD252TNFSF4 ELISA KIT; TXGP1 ELISA KIT; OX40L ELISA KIT
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Catalog
E039913
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species
Human
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GeneTNFSF4/OX40L
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 9.4pg/ml
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Intended UseHuman TNFSF4/OX40L ELISA Kit allows for the in vitro quantitative determination of TNFSF4/OX40L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human OX40L antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OX40L antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OX40L amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human OX40L can be calculated.
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