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Human USP7 ELISA Kit

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  • Alternative name

    Human deubiquitinating enzyme 7 ELISA Kit;Human Herpesvirus-associated ubiquitin-specific protease ELISA Kit;Human ubiquitin thioesterase 7 ELISA Kit;Human ubiquitin-specific-processing protease 7 ELISA Kit;Human HAUSP ELISA Kit;Human TEF1 ELISA Kit;Human ubiquitin specific peptidase 7 ELISA Kit;Human ubiquitin carboxyl-terminal hydrolase 7 ELISA Kit;Human Herpes virus-associated ubiquitin-specific protease ELISA Kit;Human ubiquitin specific peptidase 7 (herpes virus-associated) ELISA Kit;Human ubiquitin specific protease 7 (herpes virus-associated) ELISA Kit;
  • Catalog
    E040263
  • species
    Human
  • GeneUSP7
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of USP7. No significant cross-reactivity or interference between USP7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between USP7 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman USP7 ELISA Kit allows for the in vitro quantitative determination of USP7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Intended Uses: This USP7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human USP7. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||USP7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-USP7 antibody and an USP7-HRP conjugate. The assay sample and buffer are incubated together with USP7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the USP7 concentration since USP7 from samples and USP7-HRP conjugate compete for the anti-USP7 antibody binding site. Since the number of sites is limited, as more sites are occupied by USP7 from the sample, fewer sites are left to bind USP7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The USP7 concentration in each sample is interpolated from this standard curve.
  • Human Ubiquitin carboxyl-terminal hydrolase 7 Protein information
  • Uniprot ID UBP7_HUMAN
  • Uniprot AC Q93009; A6NMY8; B7Z815; H0Y3G8;
  • UniGene Hs.386939;
  • GeneID 7874
  • KEGG hsa:7874;
  • Human Ubiquitin carboxyl-terminal hydrolase 7 Protein SEQUENCE

  • SEQUENCE 1102 AA; 128302 MW; F1A5A5C421396E45 CRC64;

    MNHQQQQQQQ KAGEQQLSEP EDMEMEAGDT DDPPRITQNP VINGNVALSD

    GHNTAEEDME DDTSWRSEAT FQFTVERFSR LSESVLSPPC FVRNLPWKIM

    VMPRFYPDRP HQKSVGFFLQ CNAESDSTSW SCHAQAVLKI INYRDDEKSF

    SRRISHLFFH KENDWGFSNF MAWSEVTDPE KGFIDDDKVT FEVFVQADAP

    HGVAWDSKKH TGYVGLKNQG ATCYMNSLLQ TLFFTNQLRK AVYMMPTEGD

    DSSKSVPLAL QRVFYELQHS DKPVGTKKLT KSFGWETLDS FMQHDVQELC

    RVLLDNVENK MKGTCVEGTI PKLFRGKMVS YIQCKEVDYR SDRREDYYDI

    QLSIKGKKNI FESFVDYVAV EQLDGDNKYD AGEHGLQEAE KGVKFLTLPP

    VLHLQLMRFM YDPQTDQNIK INDRFEFPEQ LPLDEFLQKT DPKDPANYIL

    HAVLVHSGDN HGGHYVVYLN PKGDGKWCKF DDDVVSRCTK EEAIEHNYGG

    HDDDLSVRHC TNAYMLVYIR ESKLSEVLQA VTDHDIPQQL VERLQEEKRI

    EAQKRKERQE AHLYMQVQIV AEDQFCGHQG NDMYDEEKVK YTVFKVLKNS

    SLAEFVQSLS QTMGFPQDQI RLWPMQARSN GTKRPAMLDN EADGNKTMIE

    LSDNENPWTI FLETVDPELA ASGATLPKFD KDHDVMLFLK MYDPKTRSLN

    YCGHIYTPIS CKIRDLLPVM CDRAGFIQDT SLILYEEVKP NLTERIQDYD

    VSLDKALDEL MDGDIIVFQK DDPENDNSEL PTAKEYFRDL YHRVDVIFCD

    KTIPNDPGFV VTLSNRMNYF QVAKTVAQRL NTDPMLLQFF KSQGYRDGPG

    NPLRHNYEGT LRDLLQFFKP RQPKKLYYQQ LKMKITDFEN RRSFKCIWLN

    SQFREEEITL YPDKHGCVRD LLEECKKAVE LGEKASGKLR LLEIVSYKII

    GVHQEDELLE CLSPATSRTF RIEEIPLDQV DIDKENEMLV TVAHFHKEVF

    GTFGIPFLLR IHQGEHFREV MKRIQSLLDI QEKEFEKFKF AIVMMGRHQY

    INEDEYEVNL KDFEPQPGNM SHPRPWLGLD HFNKAPKRSR YTYLEKAIKI

    HN

  • UCSC uc002czk.4; human. [Q93009-1];



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