Human WIF1 ELISA Kit

Alternative name
Human WIF-1 ELISA Kit;Human wnt inhibitory factor 1 ELISA Kit;
Catalog
E040585
species
Human
GeneWIF1
SpecificityNatural and recombinant Human WIF1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseHuman WIF1 ELISA Kit allows for the in vitro quantitative determination of WIF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Human WIF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for WIF1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: G29-W379) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WIF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WIF1 amount of sample captured in plate. Background/Introduction: Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Human Wnt inhibitory factor 1 Protein information
Uniprot ID WIF1_HUMAN
Uniprot AC Q9Y5W5; Q6UXI1; Q8WVG4;
UniGene Hs.284122;
GeneID 11197
KEGG hsa:11197;
Human Wnt inhibitory factor 1 Protein SEQUENCE
SEQUENCE 379 AA; 41528 MW; 32ADFA6644833E9D CRC64;
MARRSAFPAA ALWLWSILLC LLALRAEAGP PQEESLYLWI DAHQARVLIG
FEEDILIVSE GKMAPFTHDF RKAQQRMPAI PVNIHSMNFT WQAAGQAEYF
YEFLSLRSLD KGIMADPTVN VPLLGTVPHK ASVVQVGFPC LGKQDGVAAF
EVDVIVMNSE GNTILQTPQN AIFFKTCQQA ECPGGCRNGG FCNERRICEC
PDGFHGPHCE KALCTPRCMN GGLCVTPGFC ICPPGFYGVN CDKANCSTTC
FNGGTCFYPG KCICPPGLEG EQCEISKCPQ PCRNGGKCIG KSKCKCSKGY
QGDLCSKPVC EPGCGAHGTC HEPNKCQCQE GWHGRHCNKR YEASLIHALR
PAGAQLRQHT PSLKKAEERR DPPESNYIW
UCSC uc001ssk.4; human.;

Category

Human WIF1 ELISA Kit

Human WIF-1 ELISA Kit;Human wnt inhibitory factor 1 ELISA Kit;