Human WT1 ELISA Kit

Alternative name
Human WT33 ELISA Kit;Human AWT1 ELISA Kit;Human EWS-WT1 ELISA Kit;Human GUD ELISA Kit;Human NPHS4 ELISA Kit;Human WAGR ELISA Kit;Human WIT-2 ELISA Kit;Human Wilms tumor 1 ELISA Kit;Human Wilms tumor protein ELISA Kit;Human Wilms tumor protein isoform Ex4a(+) ELISA Kit;
Catalog
E040591
species
Human
GeneWT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human WT1. No significant cross-reactivity or interference between human WT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.9 pg/ml.
Intended UseHuman WT1 ELISA Kit allows for the in vitro quantitative determination of WT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Wilms tumor protein Protein information
Uniprot ID WT1_HUMAN
Uniprot AC Q4VXV6; Q8IYZ5;
UniGene Hs.591980;
GeneID 7490
KEGG hsa:7490;
Human Wilms tumor protein Protein SEQUENCE
SEQUENCE 449 AA; 49188 MW; 11C7FA3D485096B2 CRC64;
MGSDVRDLNA LLPAVPSLGG GGGCALPVSG AAQWAPVLDF APPGASAYGS
LGGPAPPPAP PPPPPPPPHS FIKQEPSWGG AEPHEEQCLS AFTVHFSGQF
TGTAGACRYG PFGPPPPSQA SSGQARMFPN APYLPSCLES QPAIRNQGYS
TVTFDGTPSY GHTPSHHAAQ FPNHSFKHED PMGQQGSLGE QQYSVPPPVY
GCHTPTDSCT GSQALLLRTP YSSDNLYQMT SQLECMTWNQ MNLGATLKGV
AAGSSSSVKW TEGQSNHSTG YESDNHTTPI LCGAQYRIHT HGVFRGIQDV
RRVPGVAPTL VRSASETSEK RPFMCAYPGC NKRYFKLSHL QMHSRKHTGE
KPYQCDFKDC ERRFSRSDQL KRHQRRHTGV KPFQCKTCQR KFSRSDHLKT
HTRTHTGKTS EKPFSCRWPS CQKKFARSDE LVRHHNMHQR NMTKLQLAL
UCSC uc001mtl.3; human. [P19544-1];

Category

Human WT1 ELISA Kit

Human WT33 ELISA Kit;Human AWT1 ELISA Kit;Human EWS-WT1 ELISA Kit;Human GUD ELISA Kit;Human NPHS4 ELISA Kit;Human WAGR ELISA Kit;Human WIT-2 ELISA Kit;Human Wilms tumor 1 ELISA Kit;Human Wilms tumor protein ELISA Kit;Human Wilms tumor protein isoform Ex4a(+) ELISA Kit;