Human WTIP ELISA Kit

Alternative name
Human WT1-interacting protein ELISA Kit;Human Wilms tumor 1 interacting protein ELISA Kit;Human Wilms tumor protein 1-interacting protein ELISA Kit;
Catalog
E040596
species
Human
GeneWTIP
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.234ng/mL
Intended UseHuman WTIP ELISA Kit allows for the in vitro quantitative determination of WTIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WTIP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WTIP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WTIP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WTIP can be calculated.
Human Wilms tumor protein 1-interacting protein Protein information
Uniprot ID WTIP_HUMAN
Uniprot AC A6NIX2;
UniGene Hs.585010;
GeneID 126374
KEGG hsa:126374;
Human Wilms tumor protein 1-interacting protein Protein SEQUENCE
SEQUENCE 430 AA; 45124 MW; B95E162B3009B886 CRC64;
MQRSRAGADE AALLLAGLAL RELEPGCGSP GRGRRGPRPG PGDEAAPALG
RRGKGSGGPE AGADGLSRGE RGPRRAAVPE LSAQPAGSPR ASLAGSDGGG
GGGSARSSGI SLGYDQRHGS PRSGRSDPRP GPGPPSVGSA RSSVSSLGSR
GSAGAYADFL PPGACPAPAR SPEPAGPAPF PLPALPLPPG REGGPSAAER
RLEALTRELE RALEARTARD YFGICIKCGL GIYGAQQACQ AMGSLYHTDC
FTCDSCGRRL RGKAFYNVGE KVYCQEDFLY SGFQQTADKC SVCGHLIMEM
ILQALGKSYH PGCFRCSVCN ECLDGVPFTV DVENNIYCVR DYHTVFAPKC
ASCARPILPA QGCETTIRVV SMDRDYHVAC YHCEDCGLQL SGEEGRRCYP
LAGHLLCRRC HLRRLQPGPL PSPTVHVTEL
UCSC uc002nvm.4; human.;

Category

Human WTIP ELISA Kit

Human WT1-interacting protein ELISA Kit;Human Wilms tumor 1 interacting protein ELISA Kit;Human Wilms tumor protein 1-interacting protein ELISA Kit;