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Human WISP1 ELISA Kit

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  • Alternative name

    Human WISP-1 ELISA Kit;Human CCN family member 4 ELISA Kit;Human Wnt-1-induced secreted protein ELISA Kit;Human CCN4 ELISA Kit;Human WISP1c ELISA Kit;Human WISP1i ELISA Kit;Human WISP1tc ELISA Kit;Human WNT1 inducible signaling pathway protein 1 ELISA Kit;Human WNT1-inducible-signaling pathway protein 1 ELISA Kit;Human CTC-458A3.8 ELISA Kit;Human WNT1 induced secreted protein 1 ELISA Kit;Human Wnt-1 inducible signaling pathway protein 1 ELISA Kit;
  • Catalog
    E040649
  • species
    Human
  • GeneWISP1
  • SpecificityThe Human WISP1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human WISP1 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human WISP1 is typically less than 10 pg/ml.
  • Intended UseHuman WISP1 ELISA Kit allows for the in vitro quantitative determination of WISP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The MBS Bioscience Human WISP1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human WISP1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human WISP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and WISP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human WISP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WISP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
  • Human WNT1-inducible-signaling pathway protein 1 Protein information
  • Uniprot ID WISP1_HUMAN
  • Uniprot AC O95388; A8KAG6; E7EMM5; Q5JBS6; Q5JBS7; Q5JBS8; Q9HCS3;
  • UniGene Hs.492974;
  • GeneID 8840
  • KEGG hsa:8840;
  • Human WNT1-inducible-signaling pathway protein 1 Protein SEQUENCE

  • SEQUENCE 367 AA; 40331 MW; 9F29CA94D69C0502 CRC64;

    MRWFLPWTLA AVTAAAASTV LATALSPAPT TMDFTPAPLE DTSSRPQFCK

    WPCECPPSPP RCPLGVSLIT DGCECCKMCA QQLGDNCTEA AICDPHRGLY

    CDYSGDRPRY AIGVCAQVVG VGCVLDGVRY NNGQSFQPNC KYNCTCIDGA

    VGCTPLCLRV RPPRLWCPHP RRVSIPGHCC EQWVCEDDAK RPRKTAPRDT

    GAFDAVGEVE AWHRNCIAYT SPWSPCSTSC GLGVSTRISN VNAQCWPEQE

    SRLCNLRPCD VDIHTLIKAG KKCLAVYQPE ASMNFTLAGC ISTRSYQPKY

    CGVCMDNRCC IPYKSKTIDV SFQCPDGLGF SRQVLWINAC FCNLSCRNPN

    DIFADLESYP DFSEIAN

  • UCSC uc003yub.4; human. [O95388-1];



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