Human XBP1 ELISA Kit

Alternative name
Human XBP-1 ELISA Kit;Human tax-responsive element-binding protein 5 ELISA Kit;Human TREB-5 ELISA Kit;Human TREB5 ELISA Kit;Human XBP2 ELISA Kit;Human X-box binding protein 1 ELISA Kit;Human X-box-binding protein 1 ELISA Kit;
Catalog
E040704
species
Human
GeneXBP1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman XBP1 ELISA Kit allows for the in vitro quantitative determination of XBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This XBP1/TREB5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human XBP1/TREB5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY XBP1/TREB5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XBP1/TREB5 antibody and an XBP1/TREB5-HRP conjugate. The assay sample and buffer are incubated together with XBP1/TREB5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XBP1/TREB5 concentration since XBP1/TREB5 from samples and XBP1/TREB5-HRP conjugate compete for the anti-XBP1/TREB5 antibody binding site. Since the number of sites is limited, as more sites are occupied by XBP1/TREB5 from the sample, fewer sites are left to bind XBP1/TREB5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XBP1/TREB5 concentration in each sample is interpolated from this standard curve.
Human X-box-binding protein 1, luminal form Protein information
Uniprot ID XBP1_HUMAN
Uniprot AC P17861; Q8WYK6; Q969P1; Q96BD7;
UniGene Hs.437638;
GeneID 7494
KEGG hsa:7494;
Human X-box-binding protein 1, luminal form Protein SEQUENCE
SEQUENCE 261 AA; 28695 MW; A4EF69EEE0D344A6 CRC64;
MVVVAAAPNP ADGTPKVLLL SGQPASAAGA PAGQALPLMV PAQRGASPEA
ASGGLPQARK RQRLTHLSPE EKALRRKLKN RVAAQTARDR KKARMSELEQ
QVVDLEEENQ KLLLENQLLR EKTHGLVVEN QELRQRLGMD ALVAEEEAEA
KGNEVRPVAG SAESAALRLR APLQQVQAQL SPLQNISPWI LAVLTLQIQS
LISCWAFWTT WTQSCSSNAL PQSLPAWRSS QRSTQKDPVP YQPPFLCQWG
RHQPSWKPLM N
UCSC uc062cvg.1; human. [P17861-1];

Category

Human XBP1 ELISA Kit

Human XBP-1 ELISA Kit;Human tax-responsive element-binding protein 5 ELISA Kit;Human TREB-5 ELISA Kit;Human TREB5 ELISA Kit;Human XBP2 ELISA Kit;Human X-box binding protein 1 ELISA Kit;Human X-box-binding protein 1 ELISA Kit;