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Human HSPE1 ELISA Kit

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  • Alternative name

    Human HSP10 ELISA Kit;Human 10 kDa chaperonin ELISA Kit;Human chaperonin 10 ELISA Kit;Human CPN10 ELISA Kit;Human early-pregnancy factor ELISA Kit;Human EPF ELISA Kit;Human GROES ELISA Kit;Human heat shock protein family E (Hsp10) member 1 ELISA Kit;Human 10 kDa heat shock protein, mitochondrial ELISA Kit;Human heat shock 10kD protein 1 (chaperonin 10) ELISA Kit;Human heat shock 10kDa protein 1 ELISA Kit;
  • Catalog
    E041677
  • species
    Human
  • GeneHSPE1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HSPE1. No significant cross-reactivity or interference between HSPE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSPE1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman HSPE1 ELISA Kit allows for the in vitro quantitative determination of HSPE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This HSPE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSPE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||HSPE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-HSPE1 antibody and an HSPE1-HRP conjugate. The assay sample and buffer are incubated together with HSPE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSPE1 concentration since HSPE1 from samples and HSPE1-HRP conjugate compete for the anti-HSPE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPE1 from the sample, fewer sites are left to bind HSPE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPE1 concentration in each sample is interpolated from this standard curve.
  • Human 10 kDa heat shock protein, mitochondrial Protein information
  • Uniprot ID CH10_HUMAN
  • Uniprot AC P61604; O95421; Q04984; Q53X54; Q9UDH0;
  • UniGene Hs.1197;
  • GeneID 3336
  • KEGG hsa:3336;
  • Human 10 kDa heat shock protein, mitochondrial Protein SEQUENCE

  • SEQUENCE 102 AA; 10932 MW; 1F3192C81F6EDB78 CRC64;

    MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG

    SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY

    VD

  • UCSC uc002uul.4; human.;



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