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Human HSD17B1 ELISA Kit

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  • Alternative name

    Human 17-beta-hydroxysteroid dehydrogenase type 1 ELISA Kit;Human 17-beta-HSD 1 ELISA Kit;Human 20 alpha-hydroxysteroid dehydrogenase ELISA Kit;Human 20-alpha-HSD ELISA Kit;Human E2DH ELISA Kit;Human placental 17-beta-hydroxysteroid dehydrogenase ELISA Kit;Human short chain dehydrogenase/reductase family 28C member 1 ELISA Kit;Human E17KSR ELISA Kit;Human EDH17B1 ELISA Kit;Human EDH17B2 ELISA Kit;Human EDHB17 ELISA Kit;Human SDR28C1 ELISA Kit;Human 17-beta-HSD ELISA Kit;Human HSD17 ELISA Kit;Human hydroxysteroid 17-beta dehydrogenase 1 ELISA Kit;Human estradiol 17-beta-dehydrogenase 1 ELISA Kit;Human short chain dehydrogenase/reductase family 28CE, member 1 ELISA Kit;
  • Catalog
    E041761
  • species
    Human
  • GeneHSD17B1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.55ng/mL.
  • Intended UseHuman HSD17B1 ELISA Kit allows for the in vitro quantitative determination of HSD17B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Estradiol 17-beta-dehydrogenase 1 Protein information
  • Uniprot ID DHB1_HUMAN
  • Uniprot AC P14061; B3KXS1; Q2M2L8;
  • UniGene Hs.654385; Hs.655222;
  • GeneID 3292
  • KEGG hsa:3292;
  • Human Estradiol 17-beta-dehydrogenase 1 Protein SEQUENCE

  • SEQUENCE 328 AA; 34950 MW; 5B5C8909F9120832 CRC64;

    MARTVVLITG CSSGIGLHLA VRLASDPSQS FKVYATLRDL KTQGRLWEAA

    RALACPPGSL ETLQLDVRDS KSVAAARERV TEGRVDVLVC NAGLGLLGPL

    EALGEDAVAS VLDVNVVGTV RMLQAFLPDM KRRGSGRVLV TGSVGGLMGL

    PFNDVYCASK FALEGLCESL AVLLLPFGVH LSLIECGPVH TAFMEKVLGS

    PEEVLDRTDI HTFHRFYQYL AHSKQVFREA AQNPEEVAEV FLTALRAPKP

    TLRYFTTERF LPLLRMRLDD PSGSNYVTAM HREVFGDVPA KAEAGAEAGG

    GAGPGAEDEA GRGAVGDPEL GDPPAAPQ

  • UCSC uc002hzw.4; human.;



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