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Human HSD17B14 ELISA Kit

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  • Alternative name

    Human 17-beta-HSD 14 ELISA Kit;Human 17-beta-hydroxysteroid dehydrogenase DHRS10 ELISA Kit;Human Dehydrogenase/reductase SDR family member 10 ELISA Kit;Human retinal short-chain dehydrogenase/reductase retSDR3 ELISA Kit;Human short chain dehydrogenase/reductase family 47C member 1 ELISA Kit;Human DHRS10 ELISA Kit;Human SDR3 ELISA Kit;Human SDR47C1 ELISA Kit;Human retSDR3 ELISA Kit;Human hydroxysteroid 17-beta dehydrogenase 14 ELISA Kit;Human 17-beta-hydroxysteroid dehydrogenase 14 ELISA Kit;Human dehydrogenase/reductase (SDR family) member 10 ELISA Kit;Human retinal short-chain dehydrogenase/reductase 3 ELISA Kit;Human testicular tissue protein Li 52 ELISA Kit;
  • Catalog
    E041764
  • species
    Human
  • GeneHSD17B14
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.048ng/mL.
  • Intended UseHuman HSD17B14 ELISA Kit allows for the in vitro quantitative determination of HSD17B14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 17-beta-hydroxysteroid dehydrogenase 14 Protein information
  • Uniprot ID DHB14_HUMAN
  • Uniprot AC Q9BPX1; Q9UKU3;
  • UniGene Hs.18788;
  • GeneID 51171
  • KEGG hsa:51171;
  • Human 17-beta-hydroxysteroid dehydrogenase 14 Protein SEQUENCE

  • SEQUENCE 270 AA; 28317 MW; DAB68FB8CA422ABC CRC64;

    MATGTRYAGK VVVVTGGGRG IGAGIVRAFV NSGARVVICD KDESGGRALE

    QELPGAVFIL CDVTQEDDVK TLVSETIRRF GRLDCVVNNA GHHPPPQRPE

    ETSAQGFRQL LELNLLGTYT LTKLALPYLR KSQGNVINIS SLVGAIGQAQ

    AVPYVATKGA VTAMTKALAL DESPYGVRVN CISPGNIWTP LWEELAALMP

    DPRATIREGM LAQPLGRMGQ PAEVGAAAVF LASEANFCTG IELLVTGGAE

    LGYGCKASRS TPVDAPDIPS

  • UCSC uc002pkv.2; human.;



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