Human MRPS2 ELISA Kit

Alternative name
Human MRP-S2 ELISA Kit;Human S2mt ELISA Kit;Human CGI-91 ELISA Kit;Human mitochondrial ribosomal protein S2 ELISA Kit;Human 28S ribosomal protein S2, mitochondrial ELISA Kit;
Catalog
E041872
species
Human
GeneMRPS2
SpecificityThis assay has high sensitivity and excellent specificity for detection of MRPS2. No significant cross-reactivity or interference between MRPS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MRPS2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman MRPS2 ELISA Kit allows for the in vitro quantitative determination of MRPS2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This MRPS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MRPS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||MRPS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MRPS2 antibody and an MRPS2-HRP conjugate. The assay sample and buffer are incubated together with MRPS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPS2 concentration since MRPS2 from samples and MRPS2-HRP conjugate compete for the anti-MRPS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPS2 from the sample, fewer sites are left to bind MRPS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPS2 concentration in each sample is interpolated from this standard curve.
Human 28S ribosomal protein S2, mitochondrial Protein information
Uniprot ID RT02_HUMAN
Uniprot AC Q9Y399; Q5T899; Q9BSQ4;
UniGene Hs.382044;
GeneID 51116
KEGG hsa:51116;
Human 28S ribosomal protein S2, mitochondrial Protein SEQUENCE
SEQUENCE 296 AA; 33249 MW; E05A248B09AA1A8B CRC64;
MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR
ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARVH LGHKAGCRHR
FMEPYIFGSR LDHDIIDLEQ TATHLQLALN FTAHMAYRKG IILFISRNRQ
FSYLIENMAR DCGEYAHTRY FRGGMLTNAR LLFGPTVRLP DLIIFLHTLN
NIFEPHVAVR DAAKMNIPTV GIVDTNCNPC LITYPVPGND DSPLAVHLYC
RLFQTAITRA KEKRQQVEAL YRLQGQKEPG DQGPAHPPGA DMSHSL
UCSC uc004cfv.6; human.;

Category

Human MRPS2 ELISA Kit

Human MRP-S2 ELISA Kit;Human S2mt ELISA Kit;Human CGI-91 ELISA Kit;Human mitochondrial ribosomal protein S2 ELISA Kit;Human 28S ribosomal protein S2, mitochondrial ELISA Kit;