Human HSD3B1 ELISA Kit

Alternative name
Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I ELISA Kit;Human 3-beta-HSD I ELISA Kit;Human trophoblast antigen FDO161G ELISA Kit;Human 3-beta-hydroxy-5-ene steroid dehydrogenase ELISA Kit;Human progesterone reductase ELISA Kit;Human delta-5-3-ketosteroid isomerase ELISA Kit;Human 3BH ELISA Kit;Human HSDB3A ELISA Kit;Human 3BETAHSD ELISA Kit;Human HSD3B ELISA Kit;Human HSDB3 ELISA Kit;Human I ELISA Kit;Human SDR11E1 ELISA Kit;Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 ELISA Kit;Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit;Human 3-beta-hydroxy-Delta(5)-steroid dehydrogenase ELISA Kit;Human short chain dehydrogenase/reductase family 11E, member 1 ELISA Kit;Human steroid Delta-isomerase ELISA Kit;
Catalog
E041895
species
Human
GeneHSD3B1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman HSD3B1 ELISA Kit allows for the in vitro quantitative determination of HSD3B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSD3B1. During the reaction,Human HSD3B1 in the sample or standard competes with a fixed amount of biotin-labeled Human HSD3B1 for sites on a pre-coated Monoclonal antibody specific to Human HSD3B1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human HSD3B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Steroid Delta-isomerase Protein information
Uniprot ID 3BHS1_HUMAN
Uniprot AC P14060; A8K691; Q14545; Q8IV65;
UniGene Hs.364941;
GeneID 3283
KEGG hsa:3283;
Human Steroid Delta-isomerase Protein SEQUENCE
SEQUENCE 373 AA; 42252 MW; 2C76616E7EA7433A CRC64;
MTGWSCLVTG AGGFLGQRII RLLVKEKELK EIRVLDKAFG PELREEFSKL
QNKTKLTVLE GDILDEPFLK RACQDVSVII HTACIIDVFG VTHRESIMNV
NVKGTQLLLE ACVQASVPVF IYTSSIEVAG PNSYKEIIQN GHEEEPLENT
WPAPYPHSKK LAEKAVLAAN GWNLKNGGTL YTCALRPMYI YGEGSRFLSA
SINEALNNNG ILSSVGKFST VNPVYVGNVA WAHILALRAL QDPKKAPSIR
GQFYYISDDT PHQSYDNLNY TLSKEFGLRL DSRWSFPLSL MYWIGFLLEI
VSFLLRPIYT YRPPFNRHIV TLSNSVFTFS YKKAQRDLAY KPLYSWEEAK
QKTVEWVGSL VDRHKETLKS KTQ
UCSC uc001ehv.2; human.;

Category

Human HSD3B1 ELISA Kit