Human HSD17B10 ELISA Kit

Alternative name
Human 17-beta-hydroxysteroid dehydrogenase 10 ELISA Kit;Human 17-beta-HSD 10 ELISA Kit;Human 3-hydroxy-2-methylbutyryl-CoA dehydrogenase ELISA Kit;Human 3-hydroxyacyl-CoA dehydrogenase type II ELISA Kit;Human endoplasmic reticulum-associated amyloid beta-peptide-binding protein ELISA Kit;Human mitochondrial ribonuclease P protein 2 ELISA Kit;Human Mitochondrial RNase P protein 2 ELISA Kit;Human Short chain dehydrogenase/reductase family 5C member 1 ELISA Kit;Human Short-chain type dehydrogenase/reductase XH98G2 ELISA Kit;Human Type II HADH ELISA Kit;Human ERAB ELISA Kit;Human HADH2 ELISA Kit;Human MRPP2 ELISA Kit;Human SCHAD ELISA Kit;Human SDR5C1 ELISA Kit;Human XH98G2 ELISA Kit;Human 17b-HSD10 ELISA Kit;Human ABAD ELISA Kit;Human CAMR ELISA Kit;Human DUPXp11.22 ELISA Kit;Human HCD2 ELISA Kit;Human MHBD ELISA Kit;Human MRX17 ELISA Kit;Human MRX31 ELISA Kit;Human MRXS10 ELISA Kit;Human hydroxysteroid 17-beta dehydrogenase 10 ELISA Kit;Human 3-hydroxyacyl-CoA dehydrogenase type-2 ELISA Kit;Human AB-binding alcohol dehydrogenase ELISA Kit;Human amyloid-beta peptide binding alcohol dehydrogenase ELISA Kit;Human mitochondrial RNase P subunit 2 ELISA Kit;Human short chain L-3-hydroxyacyl-CoA dehydrogenase type 2 ELISA Kit;Human short chain type dehydrogenase/reductase XH98G2 ELISA Kit;
Catalog
E041903
species
Human
GeneHSD17B10
SpecificityThis assay has high sensitivity and excellent specificity for detection of HSD17B10. No significant cross-reactivity or interference between HSD17B10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSD17B10 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman HSD17B10 ELISA Kit allows for the in vitro quantitative determination of HSD17B10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This HSD17B10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSD17B10. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||HSD17B10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-HSD17B10 antibody and an HSD17B10-HRP conjugate. The assay sample and buffer are incubated together with HSD17B10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSD17B10 concentration since HSD17B10 from samples and HSD17B10-HRP conjugate compete for the anti-HSD17B10 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSD17B10 from the sample, fewer sites are left to bind HSD17B10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSD17B10 concentration in each sample is interpolated from this standard curve.
Human 3-hydroxyacyl-CoA dehydrogenase type-2 Protein information
Uniprot ID HCD2_HUMAN
Uniprot AC Q99714; Q5H927; Q6IBS9; Q8TCV9; Q96HD5;
UniGene Hs.171280;
GeneID 3028
KEGG hsa:3028;
Human 3-hydroxyacyl-CoA dehydrogenase type-2 Protein SEQUENCE
SEQUENCE 261 AA; 26923 MW; 9E74F242E3E6FEF1 CRC64;
MAAACRSVKG LVAVITGGAS GLGLATAERL VGQGASAVLL DLPNSGGEAQ
AKKLGNNCVF APADVTSEKD VQTALALAKG KFGRVDVAVN CAGIAVASKT
YNLKKGQTHT LEDFQRVLDV NLMGTFNVIR LVAGEMGQNE PDQGGQRGVI
INTASVAAFE GQVGQAAYSA SKGGIVGMTL PIARDLAPIG IRVMTIAPGL
FGTPLLTSLP EKVCNFLASQ VPFPSRLGDP AEYAHLVQAI IENPFLNGEV
IRLDGAIRMQ P
UCSC uc004dsl.2; human. [Q99714-1];

Category

Human HSD17B10 ELISA Kit