Human HMGCR ELISA Kit

Alternative name
Human HMG-CoA reductase ELISA Kit;Human LDLCQ3 ELISA Kit;Human 3-hydroxy-3-methylglutaryl-CoA reductase ELISA Kit;Human 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ELISA Kit;Human 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) ELISA Kit;Human hydroxymethylglutaryl-CoA reductase ELISA Kit;
Catalog
E041913
species
Human
GeneHMGCR
SpecificityThis assay has high sensitivity and excellent specificity for detection of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). No significant cross-reactivity or interference between 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.58ng/mL.
Intended UseHuman HMGCR ELISA Kit allows for the in vitro quantitative determination of HMGCR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase Protein information
Uniprot ID HMDH_HUMAN
Uniprot AC P04035; B7Z3Y9; Q8N190;
UniGene Hs.628096; Hs.643495;
GeneID 3156
KEGG hsa:3156;
Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase Protein SEQUENCE
SEQUENCE 888 AA; 97476 MW; 49B610DCCCFA26B6 CRC64;
MLSRLFRMHG LFVASHPWEV IVGTVTLTIC MMSMNMFTGN NKICGWNYEC
PKFEEDVLSS DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI
FSSFVFSTVV IHFLDKELTG LNEALPFFLL LIDLSRASTL AKFALSSNSQ
DEVRENIARG MAILGPTFTL DALVECLVIG VGTMSGVRQL EIMCCFGCMS
VLANYFVFMT FFPACVSLVL ELSRESREGR PIWQLSHFAR VLEEEENKPN
PVTQRVKMIM SLGLVLVHAH SRWIADPSPQ NSTADTSKVS LGLDENVSKR
IEPSVSLWQF YLSKMISMDI EQVITLSLAL LLAVKYIFFE QTETESTLSL
KNPITSPVVT QKKVPDNCCR REPMLVRNNQ KCDSVEEETG INRERKVEVI
KPLVAETDTP NRATFVVGNS SLLDTSSVLV TQEPEIELPR EPRPNEECLQ
ILGNAEKGAK FLSDAEIIQL VNAKHIPAYK LETLMETHER GVSIRRQLLS
KKLSEPSSLQ YLPYRDYNYS LVMGACCENV IGYMPIPVGV AGPLCLDEKE
FQVPMATTEG CLVASTNRGC RAIGLGGGAS SRVLADGMTR GPVVRLPRAC
DSAEVKAWLE TSEGFAVIKE AFDSTSRFAR LQKLHTSIAG RNLYIRFQSR
SGDAMGMNMI SKGTEKALSK LHEYFPEMQI LAVSGNYCTD KKPAAINWIE
GRGKSVVCEA VIPAKVVREV LKTTTEAMIE VNINKNLVGS AMAGSIGGYN
AHAANIVTAI YIACGQDAAQ NVGSSNCITL MEASGPTNED LYISCTMPSI
EIGTVGGGTN LLPQQACLQM LGVQGACKDN PGENARQLAR IVCGTVMAGE
LSLMAALAAG HLVKSHMIHN RSKINLQDLQ GACTKKTA
UCSC uc003kdp.4; human. [P04035-1];

Category

Human HMGCR ELISA Kit

Human HMG-CoA reductase ELISA Kit;Human LDLCQ3 ELISA Kit;Human 3-hydroxy-3-methylglutaryl-CoA reductase ELISA Kit;Human 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ELISA Kit;Human 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) ELISA Kit;Human hydroxymethylglutaryl-CoA reductase ELISA Kit;