Human S23 ELISA Kit;Human ribosomal protein S23 ELISA Kit;Human 40S ribosomal protein S23 ELISA Kit;Human homolog of yeast ribosomal protein S28 ELISA Kit;
Catalog
E042012
species
Human
GeneRPS23
SpecificityThis assay has high sensitivity and excellent specificity for detection of RPS23. No significant cross-reactivity or interference between RPS23 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPS23 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman RPS23 ELISA Kit allows for the in vitro quantitative determination of RPS23 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description specificalIntended Uses: This RPS23 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPS23. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||RPS23 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RPS23 antibody and an RPS23-HRP conjugate. The assay sample and buffer are incubated together with RPS23-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS23 concentration since RPS23 from samples and RPS23-HRP conjugate compete for the anti-RPS23 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS23 from the sample, fewer sites are left to bind RPS23-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS23 concentration in each sample is interpolated from this standard curve.
Human 40S ribosomal protein S23 Protein information