Human RPS3A ELISA Kit

Alternative name
Human v-fos transformation effector protein ELISA Kit;Human fte-1 ELISA Kit;Human FTE1 ELISA Kit;Human MFTL ELISA Kit;Human S3A ELISA Kit;Human ribosomal protein S3A ELISA Kit;Human 40S ribosomal protein S3a ELISA Kit;Human v-fos transformation effector protein 1 ELISA Kit;
Catalog
E042022
species
Human
GeneRPS3A
SpecificityThis assay has high sensitivity and excellent specificity for detection of RPS3A. No significant cross-reactivity or interference between RPS3A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPS3A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman RPS3A ELISA Kit allows for the in vitro quantitative determination of RPS3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: RPS3A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPS3A antibody and an RPS3A-HRP conjugate. The assay sample and buffer are incubated together with RPS3A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS3A concentration since RPS3A from samples and RPS3A-HRP conjugate compete for the anti-RPS3A antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS3A from the sample, fewer sites are left to bind RPS3A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS3A concentration in each sample is interpolated from this standard curve.
Human 40S ribosomal protein S3a Protein information
Uniprot ID RS3A_HUMAN
Uniprot AC P61247; B2R4D4; D3DP05; P33443; P49241;
UniGene Hs.356572;
GeneID 6189
KEGG hsa:6189;
Human 40S ribosomal protein S3a Protein SEQUENCE
SEQUENCE 264 AA; 29945 MW; 000037AE195F7A9D CRC64;
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT
RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITED VQGKNCLTNF
HGMDLTRDKM CSMVKKWQTM IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI
RKTSYAQHQQ VRQIRKKMME IMTREVQTND LKEVVNKLIP DSIGKDIEKA
CQSIYPLHDV FVRKVKMLKK PKFELGKLME LHGEGSSSGK ATGDETGAKV
ERADGYEPPV QESV
UCSC uc003ilz.4; human.;

Category

Human RPS3A ELISA Kit

Human v-fos transformation effector protein ELISA Kit;Human fte-1 ELISA Kit;Human FTE1 ELISA Kit;Human MFTL ELISA Kit;Human S3A ELISA Kit;Human ribosomal protein S3A ELISA Kit;Human 40S ribosomal protein S3a ELISA Kit;Human v-fos transformation effector protein 1 ELISA Kit;