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Alternative name
5-Hydroxyindoleacetic Acid ELISA Kit
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Catalog
E042058
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species
Human
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Gene5-HIAA
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SpecificityNo cross-reaction with other factors
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.938ng/ml
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Intended UseHuman 5-HIAA ELISA Kit allows for the in vitro quantitative determination of 5-HIAA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalHuman 5-HIAA ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to 5-HIAA, During the reaction,5-HIAA in the sample or standard competes with a fixed amount of biotin-labeled 5-HIAA for sites on a pre-coated Monoclonal antibody specific to 5-HIAA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5-HIAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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