Human RPL8 ELISA Kit

Alternative name
Human L8 ELISA Kit;Human ribosomal protein L8 ELISA Kit;Human 60S ribosomal protein L8 ELISA Kit;
Catalog
E042195
species
Human
GeneRPL8
SpecificityThis assay has high sensitivity and excellent specificity for detection of RPL8. No significant cross-reactivity or interference between RPL8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPL8 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman RPL8 ELISA Kit allows for the in vitro quantitative determination of RPL8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalIntended Uses: This RPL8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPL8. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||RPL8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPL8 antibody and an RPL8-HRP conjugate. The assay sample and buffer are incubated together with RPL8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPL8 concentration since RPL8 from samples and RPL8-HRP conjugate compete for the anti-RPL8 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPL8 from the sample, fewer sites are left to bind RPL8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPL8 concentration in each sample is interpolated from this standard curve.
Human 60S ribosomal protein L8 Protein information
Uniprot ID RL8_HUMAN
Uniprot AC P62917; A8K094; D3DWN2; P25120; Q567Q7; Q969V7; Q9BWQ9;
UniGene Hs.178551;
GeneID 6132
KEGG hsa:6132;
Human 60S ribosomal protein L8 Protein SEQUENCE
SEQUENCE 257 AA; 28025 MW; CE1610449749318B CRC64;
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH
DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN
VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL
PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR
GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK
TVQEKEN
UCSC uc003zeb.4; human.;

Category

Human RPL8 ELISA Kit

Human L8 ELISA Kit;Human ribosomal protein L8 ELISA Kit;Human 60S ribosomal protein L8 ELISA Kit;