Mouse Adam9 ELISA Kit

Alternative name
Mouse ADAM 9 ELISA Kit;Mouse Meltrin-gamma ELISA Kit;Mouse metalloprotease/disintegrin/cysteine-rich protein 9 ELISA Kit;Mouse myeloma cell metalloproteinase ELISA Kit;Mouse AU020942 ELISA Kit;Mouse MDC9 ELISA Kit;Mouse Mltng ELISA Kit;Mouse mKIAA0021 ELISA Kit;Mouse a disintegrin and metallopeptidase domain 9 (meltrin gamma) ELISA Kit;Mouse disintegrin and metalloproteinase domain-containing protein 9 ELISA Kit;Mouse a disintegrin and metalloproteinase domain 9 (meltrin gamma) ELISA Kit;
Catalog
E042255
species
Mouse
GeneAdam9
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADAM9. No significant cross-reactivity or interference between ADAM9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM9 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1ng/mL.
Intended UseMouse Adam9 ELISA Kit allows for the in vitro quantitative determination of Adam9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: ADAM9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM9 antibody and an ADAM9-HRP conjugate. The assay sample and buffer are incubated together with ADAM9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM9 concentration since ADAM9 from samples and ADAM9-HRP conjugate compete for the anti-ADAM9 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM9 from the sample, fewer sites are left to bind ADAM9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM9 concentration in each sample is interpolated from this standard curve.
Mouse Disintegrin and metalloproteinase domain-containing protein 9 Protein information
Uniprot ID ADAM9_MOUSE
Uniprot AC Q61072; E9QPP2; Q60618; Q61853; Q80U94;
UniGene Mm.28908;
GeneID 11502
KEGG mmu:11502;
Mouse Disintegrin and metalloproteinase domain-containing protein 9 Protein SEQUENCE
SEQUENCE 845 AA; 92079 MW; 38C40F89D4A77725 CRC64;
MGPRALSPLA SLRLRWLLAC GLLGPVLEAG RPDLEQTVHL SSYEIITPWR
LTRERREALG PSSQQISYVI QAQGKQHIIH LERNTDLLPN DFVVYTYDKE
GSLLSDHPNV QSHCHYRGYV EGVQNSAVAV SACFGLRGLL HLENASFGIE
PLHNSSHFEH IFYPMDGIHQ EPLRCGVSNR DTEKEGTQGD EEEHPSVTQL
LRRRRAVLPQ TRYVELFIVV DKERYDMMGR NQTAVREEMI RLANYLDSMY
IMLNIRIVLV GLEIWTDRNP INIIGGAGDV LGNFVQWREK FLITRWRHDS
AQLVLKKGFG GTAGMAFVGT VCSRSHAGGI NVFGQITVET FASIVAHELG
HNLGMNHDDG RECFCGAKSC IMNSGASGSR NFSSCSAEDF EKLTLNKGGS
CLLNIPKPDE AYSAPSCGNK LVDPGEECDC GTAKECEVDP CCEGSTCKLK
SFAECAYGDC CKDCQFLPGG SMCRGKTSEC DVPEYCNGSS QFCPPDVFIQ
NGYPCQNSKA YCYNGMCQYY DAQCQVIFGS KAKAAPRDCF IEVNSKGDRF
GNCGFSGSEY KKCATGNALC GKLQCENVQD MPVFGIVPAI IQTPSRGTKC
WGVDFQLGSD VPDPGMVNEG TKCDAGKICR NFQCVNASVL NYDCDIQGKC
HGHGVCNSNK NCHCEDGWAP PHCDTKGYGG SVDSGPTYNA KSTALRDGLL
VFFFLIVPLV AAAIFLFIKR DELRKTFRKK RSQMSDGRNQ ANVSRQPGDP
SISRPPGGPN VSRPPGGPGV SRPPGGPGVS RPPGGPGVSR PPPGHGNRFP
VPTYAAKQPA QFPSRPPPPQ PKISSQGNLI PARPAPAPPL YSSLT
UCSC uc009lfk.2; mouse.;

Category

Mouse Adam9 ELISA Kit