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Alternative name
Protein AMBP ELISA KIT; Alpha-1 microglycoprotein ELISA KIT; Complex-forming glycoprotein heterogeneous in chargeAMBP ELISA KIT; HCP ELISA KIT; ITIL ELISA KIT; Protein HC ELISA KIT; ITI-LC ELISA KIT
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Catalog
E042321
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species
Mouse
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GeneA1-MG
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseMouse A1-MG ELISA Kit allows for the in vitro quantitative determination of A1-MG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCardiovascular
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Product Description
specificalPrinciple of the Assay: alpha1-MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha1-MG antibody and an alpha1-MG-HRP conjugate. The assay sample and buffer are incubated together with alpha1-MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha1-MG concentration since alpha1-MG from samples and alpha1-MG-HRP conjugate compete for the anti-alpha1-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha1-MG from the sample, fewer sites are left to bind alpha1-MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha1-MG concentration in each sample is interpolated from this standard curve.
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