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Alternative name
Acetyl-CoA carboxylase ELISA KIT; Fatty acid synthetase 3 ELISA KIT; mRNA transport-defective protein 7Including the following 1 domains:Biotin carboxylase (EC:6.3.4.14)ACC1 ELISA KIT; ABP2 ELISA KIT; FAS3 ELISA KIT; MTR7 ELISA KIT; YNR016C ELISA KIT; N3175 ELISA KIT; ACC ELISA KIT
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Catalog
E042414
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species
Mouse
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GeneACC1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse ACC. No significant cross-reactivity or interference between mouse ACC and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse ACC is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
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Intended UseMouse ACC1 ELISA Kit allows for the in vitro quantitative determination of ACC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACC bound in the initial step. The color development is stopped and the intensity of the color is measured.
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