Intended UseMouse Acp1 ELISA Kit allows for the in vitro quantitative determination of Acp1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This ACP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY ACP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACP1 antibody and an ACP1-HRP conjugate. The assay sample and buffer are incubated together with ACP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACP1 concentration since ACP1 from samples and ACP1-HRP conjugate compete for the anti-ACP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACP1 from the sample, fewer sites are left to bind ACP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACP1 concentration in each sample is interpolated from this standard curve.
Mouse Low molecular weight phosphotyrosine protein phosphatase Protein information
Uniprot ID
PPAC_MOUSE
Uniprot AC
Q9D358; O88739; O88740; Q9QWF5;
UniGene
Mm.359831;
GeneID
11431
KEGG
mmu:11431;
Mouse Low molecular weight phosphotyrosine protein phosphatase Protein SEQUENCE