Mouse Inhba ELISA Kit

Alternative name
Mouse activin beta-A chain ELISA Kit;Mouse inhibin beta-A ELISA Kit;Mouse inhibin beta A chain ELISA Kit;
Catalog
E042629
species
Mouse
GeneInhba
SpecificityNatural and recombinant mouse Activin A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Inhba ELISA Kit allows for the in vitro quantitative determination of Inhba , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Activin A amount of sample captured in plate. Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factorbeta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins andactivins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Mouse Inhibin beta A chain Protein information
Uniprot ID INHBA_MOUSE
Uniprot AC Q04998;
UniGene Mm.364161; Mm.8042;
GeneID 16323
KEGG mmu:16323;
Mouse Inhibin beta A chain Protein SEQUENCE
SEQUENCE 424 AA; 47392 MW; 80C251B8754A7213 CRC64;
MPLLWLRGFL LASCWIIVRS SPTPGSEGHG SAPDCPSCAL ATLPKDGPNS
QPEMVEAVKK HILNMLHLKK RPDVTQPVPK AALLNAIRKL HVGKVGENGY
VEIEDDIGRR AEMNELMEQT SEIITFAESG TARKTLHFEI SKEGSDLSVV
ERAEVWLFLK VPKANRTRTK VTIRLFQQQK HPQGSLDTGD EAEEMGLKGE
RSELLLSEKV VDARKSTWHI FPVSSSIQRL LDQGKSSLDV RIACEQCQES
GASLVLLGKK KKKEVDGDGK KKDGSDGGLE EEKEQSHRPF LMLQARQSED
HPHRRRRRGL ECDGKVNICC KKQFFVSFKD IGWNDWIIAP SGYHANYCEG
ECPSHIAGTS GSSLSFHSTV INHYRMRGHS PFANLKSCCV PTKLRPMSML
YYDDGQNIIK KDIQNMIVEE CGCS
UCSC uc007pnw.1; mouse.;

Category

Mouse Inhba ELISA Kit

Mouse activin beta-A chain ELISA Kit;Mouse inhibin beta-A ELISA Kit;Mouse inhibin beta A chain ELISA Kit;