Mouse Adk ELISA Kit

Alternative name
Mouse Ak ELISA Kit;Mouse adenosine 5'-phosphotransferase ELISA Kit;Mouse 2310026J05Rik ELISA Kit;Mouse 5033405D03Rik ELISA Kit;Mouse AI255373 ELISA Kit;Mouse AI987814 ELISA Kit;Mouse adenosine kinase ELISA Kit;
Catalog
E042830
species
Mouse
GeneAdk
SpecificityThis assay has high sensitivity and excellent specificity for detection of Adenosine Kinase (ADK). No significant cross-reactivity or interference between Adenosine Kinase (ADK) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseMouse Adk ELISA Kit allows for the in vitro quantitative determination of Adk , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine Kinase (ADK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine Kinase (ADK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine Kinase (ADK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine Kinase (ADK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Adenosine kinase Protein information
Uniprot ID ADK_MOUSE
Uniprot AC P55264; Q6JAM3; Q91VJ3;
UniGene Mm.188734;
GeneID 11534
KEGG mmu:11534;
Mouse Adenosine kinase Protein SEQUENCE
SEQUENCE 361 AA; 40149 MW; 73D5D0451B606757 CRC64;
MAAADEPKPK KLKVEAPQAL SENVLFGMGN PLLDISAVVD KDFLDKYSLK
PNDQILAEDK HKELFDELVK KFKVEYHAGG STQNSMKVAQ WLIQEPHKAA
TFFGCIGIDK FGEILKRKAA DAHVDAHYYE QNEQPTGTCA ACITGGNRSL
VANLAAANCY KKEKHLDLER NWVLVEKARV YYIAGFFLTV SPESVLKVAR
YAAENNRVFT LNLSAPFISQ FFKEALMDVM PYVDILFGNE TEAATFAREQ
GFETKDIKEI AKKAQALPKV NSKRQRTVIF TQGRDDTIVA AENDVTAFPV
LDQNQEEIID TNGAGDAFVG GFLSQLVSDK PLTECIRAGH YAASVIIRRT
GCTFPEKPDF H
UCSC uc007sle.2; mouse. [P55264-2];

Category

Mouse Adk ELISA Kit

Mouse Ak ELISA Kit;Mouse adenosine 5'-phosphotransferase ELISA Kit;Mouse 2310026J05Rik ELISA Kit;Mouse 5033405D03Rik ELISA Kit;Mouse AI255373 ELISA Kit;Mouse AI987814 ELISA Kit;Mouse adenosine kinase ELISA Kit;