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Alternative name
Adiponectin ELISA KIT; 30 kDa adipocyte complement-related protein ELISA KIT; Adipocyte complement-related 30 kDa protein ELISA KIT; ACRP30 ELISA KIT; Adipocyte, C1q and collagen domain-containing protein ELISA KIT; Adipocyte-specific protein AdipoQAdipoq ELISA KIT; Acdc ELISA KIT; Acrp30 ELISA KIT; Apm1 ELISA KIT; ACRP30 ELISA KIT
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Catalog
E042933
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species
Mouse
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GeneADP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse ADIPOQ. No significant cross-reactivity or interference between Mouse ADIPOQ and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.039 ng/ml
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Intended UseMouse ADP ELISA Kit allows for the in vitro quantitative determination of ADP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntroduction: Adiponectin (also referred to as Acrp30, apM1) is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid catabolism. Adiponectin is exclusively secreted from adipose tissue into the bloodstream and is very abundant in plasma relative to many hormones. Levels of the hormone are inversely correlated with body fat percentage in adults, while the association in infants and young children is more unclear. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin is a 244-amino-acid-long polypeptide. There are four distinct regions of adiponectin. The first is a short signal squence that targets the hormone for secretion outside the cell; next is a short region that varies between species; the third is a 65-amino acid region with similarity to collagenous proteins; the last is a globular domain. Overall this gene shows similarity to the complement 1Q factors. However, when the 3-dimensional structure of the globular region was determined, a striking similarity to TNFalpha was observed, despite unrelated protein sequences. Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein at around 5-10 ug/mL. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ADP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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