Mouse Adm ELISA Kit

Alternative name
Mouse AM ELISA Kit;Mouse ProAM N-terminal 20 peptide ELISA Kit;Mouse PAMP ELISA Kit;Mouse ProAM-N20 ELISA Kit;Mouse adrenomedullin ELISA Kit;Mouse ADM ELISA Kit;
Catalog
E042941
species
Mouse
GeneAdm
SpecificityThis kit recognizes natural and recombinant Mouse ADM. No significant cross-reactivity or interference between Mouse ADM and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Mouse ADM is 9.375pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
Intended UseMouse Adm ELISA Kit allows for the in vitro quantitative determination of Adm , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADM. You can calculate the concentration of ADM in the samples by comparing the OD of the samples to the standard curve.
Mouse Proadrenomedullin N-20 terminal peptide Protein information
Uniprot ID ADML_MOUSE
Uniprot AC P97297; P97453; Q6GTK2;
UniGene Mm.1408;
GeneID 11535
KEGG mmu:11535;
Mouse Proadrenomedullin N-20 terminal peptide Protein SEQUENCE
SEQUENCE 184 AA; 20750 MW; C88C9903C479C898 CRC64;
MKLVSITLML LGSLAFLGAD TAGPDTPSQF RKKWNKWALS RGKRELQASS
SYPTGLADET TVPTQTLDPF LDEQNTTGPL QASNQSEAHI RVKRYRQSMN
QGSRSNGCRF GTCTFQKLAH QIYQLTDKDK DGMAPRNKIS PQGYGRRRRR
SLLEVLRSRT VESSQEQTHT APGPWAHISR LFRI
UCSC uc009jfj.1; mouse.;

Category

Mouse Adm ELISA Kit

Mouse AM ELISA Kit;Mouse ProAM N-terminal 20 peptide ELISA Kit;Mouse PAMP ELISA Kit;Mouse ProAM-N20 ELISA Kit;Mouse adrenomedullin ELISA Kit;Mouse ADM ELISA Kit;