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Mouse Acer1 ELISA Kit

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  • Alternative name

    Mouse alkCDase 1 ELISA Kit;Mouse alkaline cdase 1 ELISA Kit;Mouse maCER1 ELISA Kit;Mouse acylsphingosine deacylase 3 ELISA Kit;Mouse N-acylsphingosine amidohydrolase 3 ELISA Kit;Mouse Asah3 ELISA Kit;Mouse 2310024P18Rik ELISA Kit;Mouse AI662009 ELISA Kit;Mouse Alkcdase1 ELISA Kit;Mouse Cer1 ELISA Kit;Mouse alkaline ceramidase 1 ELISA Kit;
  • Catalog
    E043228
  • species
    Mouse
  • GeneAcer1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACER1. No significant cross-reactivity or interference between ACER1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACER1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Acer1 ELISA Kit allows for the in vitro quantitative determination of Acer1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: ACER1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER1 antibody and an ACER1-HRP conjugate. The assay sample and buffer are incubated together with ACER1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER1 concentration since ACER1 from samples and ACER1-HRP conjugate compete for the anti-ACER1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER1 from the sample, fewer sites are left to bind ACER1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER1 concentration in each sample is interpolated from this standard curve.
  • Mouse Alkaline ceramidase 1 Protein information
  • Uniprot ID ACER1_MOUSE
  • Uniprot AC Q8R4X1; A1L3S2;
  • UniGene Mm.218784;
  • GeneID 171168
  • KEGG mmu:171168;
  • Mouse Alkaline ceramidase 1 Protein SEQUENCE

  • SEQUENCE 273 AA; 32082 MW; 0B84D015D7870219 CRC64;

    MHVPGTRAKM SSIFAYQSSE VDWCESNFQH SELVAEFYNT FSNVFFLIFG

    PLMMFLMHPY AQKRTRCFYG VSVLFMLIGL FSMYFHMTLS FLGQLLDEIS

    ILWLLASGYS VWLPRCYFPK FVKGNRFYFS CLVTITTIIS TFLTFVKPTV

    NAYALNSIAI HILYIVRTEY KKIRDDDLRH LIAVSVVLWA AALTSWISDR

    VLCSFWQRIH FYYLHSIWHV LISITFPYGI VTMALVDAKY EMPDKTLKVH

    YWPRDSWVIG LPYVEIQEND KNC

  • UCSC uc008ddl.1; mouse.;



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