Mouse Alpl ELISA Kit

Alternative name
Mouse AP-TNAP ELISA Kit;Mouse TNSALP ELISA Kit;Mouse Alkaline phosphatase 2 ELISA Kit;Mouse Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit;Mouse Akp-2 ELISA Kit;Mouse Akp2 ELISA Kit;Mouse ALP ELISA Kit;Mouse APTNAP ELISA Kit;Mouse TNAP ELISA Kit;Mouse alkaline phosphatase, liver/bone/kidney ELISA Kit;Mouse alkaline phosphatase, tissue-nonspecific isozyme ELISA Kit;Mouse alkaline phosphatase 2, liver ELISA Kit;
Catalog
E043237
species
Mouse
GeneAlpl
SpecificityThis assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). No significant cross-reactivity or interference between Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseMouse Alpl ELISA Kit allows for the in vitro quantitative determination of Alpl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Liver/Bone/Kidney (ALPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Alkaline phosphatase, tissue-nonspecific isozyme Protein information
Uniprot ID PPBT_MOUSE
Uniprot AC P09242; Q6P1B0;
UniGene Mm.288186;
GeneID 11647
KEGG mmu:11647;
Mouse Alkaline phosphatase, tissue-nonspecific isozyme Protein SEQUENCE
SEQUENCE 524 AA; 57514 MW; 59D99110C60FA050 CRC64;
MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV
AKNVIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH
EAVEMDQAIG KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
GSGSAPSPGA LLLPLAVLSL RTLF
UCSC uc008vjr.3; mouse.;

Category

Mouse Alpl ELISA Kit