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Alternative name
Alpha-1-antitrypsin ELISA KIT; Alpha-1 protease inhibitor ELISA KIT; Alpha-1-antiproteinase ELISA KIT; Serpin A1SERPINA1 ELISA KIT; AAT ELISA KIT; PI ELISA KIT; SPAAT ELISA KIT
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Catalog
E043327
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species
Mouse
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Genea1AT
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT).
No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 13.2ng/mL.
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Intended UseMouse a1AT ELISA Kit allows for the in vitro quantitative determination of a1AT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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