Mouse Adra1d ELISA Kit

Alternative name
Mouse Alpha-1A adrenergic receptor ELISA Kit;Mouse Alpha-1D adrenoreceptor ELISA Kit;Mouse Alpha-1D adrenoceptor ELISA Kit;Mouse Adra1a ELISA Kit;Mouse Gpcr8 ELISA Kit;
Catalog
E043343
species
Mouse
GeneAdra1d
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADRA1D. No significant cross-reactivity or interference between ADRA1D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADRA1D and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Adra1d ELISA Kit allows for the in vitro quantitative determination of Adra1d , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalIntended Uses: This ADRA1D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADRA1D. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ADRA1D ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADRA1D antibody and an ADRA1D-HRP conjugate. The assay sample and buffer are incubated together with ADRA1D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRA1D concentration since ADRA1D from samples and ADRA1D-HRP conjugate compete for the anti-ADRA1D antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRA1D from the sample, fewer sites are left to bind ADRA1D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRA1D concentration in each sample is interpolated from this standard curve.
Mouse Alpha-1D adrenergic receptor Protein information
Uniprot ID ADA1D_MOUSE
Uniprot AC P97714; Q61619;
UniGene Mm.389380;
Mouse Alpha-1D adrenergic receptor Protein SEQUENCE
SEQUENCE 562 AA; 59858 MW; 6CF15515B5F7FA47 CRC64;
MTFRDILSVT FEGPRASSST GGSGAGGGAG TVGPEGPAVG GVPGATGGSA
VVGTGSGEDN QSSTAEAGAA ASGEVNGSAA VGGLVVSAQG VGVGVFLAAF
ILTAVAGNLL VILSVACNRH LQTVTNYFIV NLAVADLLLS AAVLPFSATM
EVLGFWPFGR TFCDVWAAVD VLCCTASILS LCTISVDRYV GVRHSLKYPA
IMTERKAAAI LALLWAVALV VSVGPLLGWK EPVPPDERFC GITEEVGYAI
FSSVCSFYLP MAVIVVMYCR VYVVARSTTR SLEAGIKREP GKASEVVLRI
HCRGAATSAK GNPGTQSSKG HTLRSSLSVR LLKFSREKKA AKTLAIVVGV
FVLCWFPFFF VLPLGSLFPQ LKPSEGVFKV IFWLGYFNSC VNPLIYPCSS
REFKRAFLRL LRCQCRRRRR RLWPSLRPPL ASLDRRPALR LCPQPAHRTP
RGSPSPHCTP RPGLRRHAGG AGFGLRPSKA SLRLREWRLL GPLQRPTTQL
RAKVSSLSHK FRSGGARRAE TACALRSEVE AVSLNVPQDG AEAVICQAYE
PGDLSNLRET DI

Category

Mouse Adra1d ELISA Kit

Mouse Alpha-1A adrenergic receptor ELISA Kit;Mouse Alpha-1D adrenoreceptor ELISA Kit;Mouse Alpha-1D adrenoceptor ELISA Kit;Mouse Adra1a ELISA Kit;Mouse Gpcr8 ELISA Kit;