Mouse Amy1 ELISA Kit

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  • Alternative name

    Mouse 1,4-alpha-D-glucan glucanohydrolase 1 ELISA Kit;Mouse salivary and hepatic alpha-amylase ELISA Kit;Mouse Amy-1-a ELISA Kit;Mouse Amy1a ELISA Kit;Mouse Amy-1 ELISA Kit;Mouse C030014B17Rik ELISA Kit;Mouse amylase 1, salivary ELISA Kit;Mouse alpha-amylase 1 ELISA Kit;

  • Catalog
    E043386
  • species
    Mouse
  • GeneAmy1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<46.9pg/ml
  • Intended UseMouse Amy1 ELISA Kit allows for the in vitro quantitative determination of Amy1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Amy1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Amy1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Amy1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Amy1 can be calculated.
  • Mouse Alpha-amylase 1 Protein information
  • Uniprot ID AMY1_MOUSE
  • Uniprot AC P00687; Q921Y7;
  • UniGene Mm.439727;
  • GeneID 11722
  • KEGG mmu:11722;
  • Mouse Alpha-amylase 1 Protein SEQUENCE
  • SEQUENCE 511 AA; 57644 MW; CC5399F4C23E1105 CRC64;

    MKFFLLLSLI GFCWAQYDPH TQYGRTAIVH LFEWRWVDIA KECERYLAPN

    GFAGVQVSPP NENIVVHSPS RPWWERYQPI SYKICSRSGN EDEFRDMVNR

    CNNVGVRIYV DAVINHMCGV GAQAGQSSTC GSYFNPNNRD FPGVPYSGFD

    FNDGKCRTAS GGIENYQDAA QVRDCRLSGL LDLALEKDYV RTKVADYMNH

    LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNTKWFSQG SRPFIFQEVI

    DLGGEAVSSN EYFGNGRVTE FKYGAKLGKV MRKWDGEKMS YLKNWGEGWG

    LMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF

    TRVMSSYYWP RNFQNGKDVN DWVGPPNNNG KTKEVSINPD STCGNDWICE

    HRWRQIRNMV AFRNVVNGQP FANWWDNDSN QVAFGRGNKG FIVFNNDDWA

    LSETLQTGLP AGTYCDVISG DKVDGNCTGI KVYVGNDGKA HFSISNSAED

    PFIAIHAESK I

  • UCSC uc008rba.2; mouse.;



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