Mouse Scnn1b ELISA Kit

Alternative name
Mouse beta-NaCH ELISA Kit;Mouse epithelial Na(+) channel subunit beta ELISA Kit;Mouse beta-ENaC ELISA Kit;Mouse nonvoltage-gated sodium channel 1 subunit beta ELISA Kit;Mouse SCNEB ELISA Kit;Mouse sodium channel, nonvoltage-gated 1 beta ELISA Kit;Mouse amiloride-sensitive sodium channel subunit beta ELISA Kit;Mouse ENaC beta ELISA Kit;
Catalog
E043599
species
Mouse
GeneScnn1b
SpecificityThis assay has high sensitivity and excellent specificity for detection of SCNN1beta. No significant cross-reactivity or interference between SCNN1beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCNN1beta and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Scnn1b ELISA Kit allows for the in vitro quantitative determination of Scnn1b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This SCNN1beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SCNN1beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||SCNN1beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SCNN1beta antibody and an SCNN1beta-HRP conjugate. The assay sample and buffer are incubated together with SCNN1beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCNN1beta concentration since SCNN1beta from samples and SCNN1beta-HRP conjugate compete for the anti-SCNN1beta antibody binding site. Since the number of sites is limited, as more sites are occupied by SCNN1beta from the sample, fewer sites are left to bind SCNN1beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCNN1beta concentration in each sample is interpolated from this standard curve.
Mouse Amiloride-sensitive sodium channel subunit beta Protein information
Uniprot ID SCNNB_MOUSE
Uniprot AC Q9WU38;
UniGene Mm.7709;
GeneID 20277
KEGG mmu:20277;
Mouse Amiloride-sensitive sodium channel subunit beta Protein SEQUENCE
SEQUENCE 638 AA; 72197 MW; F502A510BFE65392 CRC64;
MPVKKYLLKC LHRLQKGPGY TYKELLVWYC NNTNTHGPKR IICEGPKKKA
MWFLLTLLFA CLVCWQWGVF IQTYLSWEVS VSLSMGFKTM NFPAVTVCNS
SPFQYSKVKH LLKDLDELME AVLEKILAPE ASHSNTTRTL NFTIWNHTPL
VLIDERNPDH PVVLNLFGDS HNSSNPAPGS TCNAQGCKVA MRLCSANGTV
CTLRNFTSAT QAVTEWYILQ ATNIFSQVLP QDLVGMGYAP DRIILACLFG
TEPCSHRNFT PIFYPDYGNC YIFNWGMTEE TLPSANPGTE FGLKLILDIG
QEDYVPFLAS TAGARLMLHE QRTYPFIREE GIYAMAGTET SIGVLVDKLQ
RKGEPYSPCT MNGSDVAIKN LYSVYNTTYS IQACLHSCFQ DHMIRNCSCG
HYLYPLPEGE KYCNNRDFPD WAYCYLNLQM SVTQRETCLS MCKESCNDTQ
YKMTISMADW PSEASEDWIL HVLSQERDQS SNITLSRKGI VKLNIYFQEF
NYRTIEESPA NNIVWLLSNL GGQFGFWMGG SVLCLIEFGE IIIDFIWITI
IKLVASCKGL RRRRPQAPYT GPPPTVAELV EAHTNFGFQP DTTSCRPHGE
VYPDQQTLPI PGTPPPNYDS LRLQPLDTME SDSEVEAI
UCSC uc009jnx.2; mouse.;

Category

Mouse Scnn1b ELISA Kit