Mouse Amt ELISA Kit

Alternative name
Mouse glycine cleavage system T protein ELISA Kit;Mouse GCVT ELISA Kit;Mouse EG434437 ELISA Kit;Mouse aminomethyltransferase ELISA Kit;Mouse aminomethyltransferase, mitochondrial ELISA Kit;
Catalog
E043622
species
Mouse
GeneAmt
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseMouse Amt ELISA Kit allows for the in vitro quantitative determination of Amt , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse AMT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse AMT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse AMT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse AMT can be calculated.
Mouse Aminomethyltransferase, mitochondrial Protein information
Uniprot ID GCST_MOUSE
Uniprot AC Q8CFA2;
UniGene Mm.390225;
GeneID 434437
KEGG mmu:434437;
Mouse Aminomethyltransferase, mitochondrial Protein SEQUENCE
SEQUENCE 403 AA; 44009 MW; 4FDEC0DC81492167 CRC64;
MHRIVSVVAP LGFRLQAQPL VQSRPLSSVQ DVLRRTPLYD FHLAHGGKMV
AFAGWSLPVQ YRDSHVDSHL HTRRHCSLFD VSHMLQTKIF GCDRVKLLES
VVVGDIAELR PNQGTLSLFT NEAGGILDDL IVSNTSEGHL YVVSNAGCRD
KDLALMQDKV KEFQNRGLDV GLEVVENALL ALQGPTATQV LQAGVTDDMK
KLPFMTSAVM EVFGVSGCRV TRCGYTGEDG VEISVPATGA VHLATTLLKN
PEVKLAGLAA RDSLRLEAGL CLYGNDIDEH TTPVEGSLSW TLGKRRRIAM
DFPGAKIIVP QLKGEVQRRR VGLICEGAPV RAHSPILNTE GTVIGTVTSG
CPSPSLKKNV AMGYVPFKYS RPGTQLLVEV RRKQQMTVVS KMPFVPTNYY
TLK
UCSC uc009rpc.1; mouse.;

Category

Mouse Amt ELISA Kit

Mouse glycine cleavage system T protein ELISA Kit;Mouse GCVT ELISA Kit;Mouse EG434437 ELISA Kit;Mouse aminomethyltransferase ELISA Kit;Mouse aminomethyltransferase, mitochondrial ELISA Kit;