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Alternative name
Acetylcholinesterase ELISA KIT; ACHE ELISA KIT; AChE ELISA KIT
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Catalog
E044117
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species
Mouse
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GeneAchEAb
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 ng/mL.
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Intended UseMouse AchEAb ELISA Kit allows for the in vitro quantitative determination of AchEAb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyNeuroscience
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Product Description
specificalIntended Uses: This AchEAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AchEAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: AchEAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti-acetylcholinesterase antigen and an AchEAb-HRP conjugate. The assay sample and buffer are incubated together with AchEAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AchEAb concentration since AchEAb from samples and AchEAb-HRP conjugate compete for the Anti-acetylcholinesterase antigen binding site. Since the number of sites is limited, as more sites are occupied by AchEAb from the sample, fewer sites are left to bind AchEAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AchEAb concentration in each sample is interpolated from this standard curve.
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