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Catalog
E044170
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species
Mouse
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GeneACCPA
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse ACCPA. No significant cross-reactivity or interference between Mouse ACCPA and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse ACCPA ELISA Kit allows for the in vitro quantitative determination of ACCPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Mouse ACCPA. Standards or samples are then added to the appropriate micro ELISA plate wells and combined to the specific antigen. Then a biotinylated detection antigen specific for Mouse ACCPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACCPA, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turn yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ACCPA. You can calculate the concentration of Mouse ACCPA in the samples by comparing the OD of the samples to the standard curve.
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